Characteristics

Networking of gene importer systems

General intelligent instrumentation has two-way communication functions, but this two-way communication function is far from the true network communication.

Along with the rapid development of network technology, Internet technology enables the gene importer to realize network at the same time on the basis of intelligentization, so that the on-site measurement and control parameters are close to the network and have the necessary information processing functions.

New Models：

SCIENTZ-2CS

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Along with the rapid development of network technology, Internet technology enables the gene importer to realize network at the same time on the basis of intelligentization, so that the on-site measurement and control parameters are close to the network and have the necessary information processing functions.

Performance Features:

■Integration of the whole machine, simple operation, direct display
■The microprocessor control of pulse discharge
■The log storage
■Unlimited custom program storage
■Simple operation, short conversion time
■High conversion efficiency
■repeat ability

Import method

Sticky end connection

Flat end connection

Artificial joint method

Homologous polytail junction

 Main Technical Parameters:

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The CRY-3B Integrated Electric Cell Fusion Instrument is suitable for cell hybridization and cell fusion experiments, enabling direct observation under an inverted microscope. It is widely applied in microbiology research, veterinary medicine, and bioengineering fields. Compared with conventional methods, it offers enhanced safety, ease of use, and efficiency. Both planar electrodes and needle electrodes are available for selection.

Core Performance Features

Dual Functions: Capable of both cell electrofusion and electroporation.

Wide Parameter Range: Flexible adjustment of voltage and pulse time to meet diverse experimental needs.

Fast & Efficient: Completes cell arrangement, fusion, and post-processing in just a few seconds; simple and rapid post-operation steps.

Intuitive Operation: LCD touch screen for easy and quick data setting and operation.

Applications

Cell fusion (fusion process observable under inverted microscope)

Nuclear transfer

Embryo manipulation

Hybridoma production

Plant protoplast fusion

Chicken embryo gene delivery

Live cell gene/drug transfection

Main Technical Parameters

Optional Microscope Slide Electrodes

Maintenance of Optional Electrodes

Clean with mild detergent, ethanol, or sterilize via ethylene oxide.

Cell Fusion Induction Methods

There are three main methods for inducing cell fusion:

Biological Methods (Virus-mediated): Viruses such as Sendai virus, parainfluenza virus, and Newcastle disease virus possess fusion proteins that mediate fusion between viruses and host cells, as well as cell-to-cell fusion. Live viruses can be used to induce cell fusion.

Chemical Methods (PEG-mediated): Polyethylene glycol (PEG) induces changes in the arrangement of membrane lipid molecules. After removing PEG, the plasma membrane restores its ordered structure, and adjacent cells can fuse during this recovery process.

Physical Methods (Electrical/laser-mediated): Electrical excitation or laser treatment disrupts the plasma membrane temporarily, allowing cell fusion when the membrane repairs.

Application Value of Cell Fusion Technology

Cell fusion technology is not only widely used in basic research but also holds significant practical value:

Plant Breeding: Successful cultivation of hybrid crops such as radish-cabbage, Nicotiana glauca × Nicotiana langsdorffii, and tomato-potato.

Agriculture, Industry, and Medicine: Achieved groundbreaking research results, with continuously expanding application fields.

Animal Somatic Hybridization:

Gene mapping: Correlates the presence/absence of chromosomes or fragments in hybrid cells with trait expression, enabling gene localization. For example, Weise and Green (1967) discovered preferential loss of human chromosomes in human-mouse hybrid cells, facilitating gene mapping on human chromosomes. Ruddle et al. (1970) systematically used fusion cells for human gene mapping.

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The digital electrophoresis instrument is widely used for cell hybridization, fusion, and gene introduction.

 Main Technical Parameters:

Selection of electron electrophoresis instrument

1.0.4 cm pitch electric shock cup: wide spacing, low field strength, applied to mammalian cells and other eukaryotic cells (200ul)

2.0.2 cm pitch electric shock cup: narrow pitch, high field strength, applied to yeast, bacteria and other eukaryotic cells (100ul)

3.0.1 cm pitch electric shock cup: narrowest pitch, very high field strength, shallow bottom for small volume samples (40–80 μl) for yeast and bacterial transformation

4. Strong field electric field strength formula: E=U/d (d is the distance between two points along the field strength direction, electric cup is this formula)

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