Purpose

Grinding brain tissue

Laboratory supplies

Sample: mouse brain tissue

Instrument: High-throughput tissue grinder (Xinzhi)

Consumables: special grinding tube (Xinzhi, 2ml), grinding beads (Xinzhi, 5mm, 2)

Experimental procedure

1. Prepare 2ml (if the sample size is large, select 5ml stainless steel grinding tube), clean the grinding tube and grinding ball, and sterilize the enzyme;

2. Cut a small piece of brain tissue that needs to be ground into a prepared grinding tube, add 2 grinding beads, cover the lid; add 300 ml of liquid nitrogen, then put it into a 2 ml adapter, the adapter can be placed in advance The -4 ° C refrigerator is pre-cooled and relatively controlled during the dry grinding process.

3. Load the adapter into the new mill);

4. Set 40hz, 60s, start the instrument until the end of the grinding process.

Experimental result

Before treatment                            After processing

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The high-throughput tissue grinder is a common tool for laboratory sample preparation. It pulverizes, mixes, homogenizes, and breaks the sample by twisting and oscillating inside the sample tube through tungsten carbide pellets or stainless steel beads.

It can be used for rapid pulverization and homogenization of samples such as hard, soft and elastic. It meets the requirements of physical and chemical analysis laboratories, and can be equipped with grinding tanks of different volumes and materials for dry grinding, wet grinding and freeze grinding.

Cell disruption and DNA/RNA extraction are also available, and are widely used in agriculture, biomedicine, food, quality inspection, and universities.

Features:

1. It can meanwhile grind 2*96 pairs of samples in 3 minutes.

2. A free choice in dry grinding,wet grinding,mixing and homogenizing treatment.

3. Sample categories provided :plant tissues ,animal tissues, cells, bacteria and yeast,etc.

4. For large-throughput sample extraction of nucleic acid, the grinder can greatly enhance the efficiency and

quality of grinding.

Technical parameters:

1.Grinding Frequency: 3-30times/sec

2.Timing : 1-99 minutes

3.Rated: 375W

4.Sample Size: with four sets of accessories :0.2-0.5ml/192 holes,

1ml/72 holes,2ml/64 holes and 5ml/48 holes

5.Dimensions: L520×W365×H505 mm

6.Supplied with a hundred of steel balls for free.

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High-throughput tissue grinder can grind 4× 96 pcs samples fast and efficient at the same time within 3 minutes. Can do dry grinding, wet grinding and mixing and homogenization. Sample categories include: plant tissue (rice, corn, wheat, beans, peanuts,) animal tissues (muscle, intestine, heart, liver, lung, skin, bones, etc.), cells, bacteria, yeast and all kinds of herbs, etc. To extract the nucleic acid from large high-throughput samples, greatly enhance the efficiency and quality of grinding. The labor cost is reduced and the labor intensity of the operator is lightened

 Principle:

A small steel ball (or zirconia ball) is added to a grinding tank or centrifuge tube, and the grinding tank or adapter is vertically vibrating. The ball is struck at high speed under high frequency vibration to grind the sample.

It adopts grinding ball to grind the sample, which make the grinding of the sample more uniform and sufficient than the traditional grinding method. Using high throughput tissue grinder, can be used as a homogenizer in grinding tank adding ball and cell beads broken the samples which it is difficult to crush by ultrasonic homogenizer, quickly dispersed samples with high density and small steel balls or zirconia beads.

Scientz-48 HIGH-THROUGHPUT TISSUE GRINDER

Main Technical Parameters：

Power Supply：220V/50Hz

Rated Power：180W

Timing：1（S）-9999（S）

Frequency Setting：10-70Hz，300-2100times/min

Clamp Stroke：34mm（vertical）

Sample Capacity：2ml Adaptor 48hole、5ml Adaptor12 hole

Display：7inch TFT Touch Screen

Protection: Open lid protection，Emergency braking function

Dimension：L369*W360*H520mm

Accompany accessories: 500pcs 3mm stainless steel ball and 500pcs 6mm stainless steel ball

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High-throughput tissue grinder can grind 4× 96 pcs samples fast and efficient at the same time within 3 minutes. Can do dry grinding, wet grinding and mixing and homogenization. Sample categories include: plant tissue (rice, corn, wheat, beans, peanuts,) animal tissues (muscle, intestine, heart, liver, lung, skin, bones, etc.), cells, bacteria, yeast and all kinds of herbs, etc. To extract the nucleic acid from large high-throughput samples, greatly enhance the efficiency and quality of grinding. The labor cost is reduced and the labor intensity of the operator is lightened

Principle:

A small steel ball (or zirconia ball) is added to a grinding tank or centrifuge tube, and the grinding tank or adapter is vertically vibrating. The ball is struck at high speed under high frequency vibration to grind the sample.

It adopts grinding ball to grind the sample, which make the grinding of the sample more uniform and sufficient than the traditional grinding method. Using high throughput tissue grinder, can be used as a homogenizer in grinding tank adding ball and cell beads broken the samples which it is difficult to crush by ultrasonic homogenizer, quickly dispersed samples with high density and small steel balls or zirconia beads.

Scientz-48 HIGH-THROUGHPUT TISSUE GRINDER

Main Technical Parameters：

Power Supply：220V/50Hz

Rated Power：180W

Timing：1（S）-9999（S）

Frequency Setting：10-70Hz，300-2100times/min

Clamp Stroke：34mm（vertical）

Sample Capacity：2ml Adaptor 48hole、5ml Adaptor12 hole

Display：7inch TFT Touch Screen

Protection: Open lid protection，Emergency braking function

Dimension：L369*W360*H520mm

Accompany accessories: 500pcs 3mm stainless steel ball and 500pcs 6mm stainless steel ball

The post 10-70HZ High Quality High Throughput Tissuelyser appeared first on Keylaser Armenia.

Main Features:

1.Not easy to cause cross-contamination, using closed disposable centrifuge tubes;

2.Suitable for a variety of samples, plant samples (rice, corn, wheat, beans, peanuts, etc.), animal samples (muscle, small intestine, heart, liver, lung, fur, bones, etc.), bacteria and yeast samples.

With the use of silica and zirconia grinding media, microbial samples can achieve better grinding results;

3.During the homogenization process, the speed can be easily adjusted;

4.Rapidly disperse the sample with high-density glass beads or zirconia beads, the sample temperature does not rise during the homogenization process

Technical parameters:

1.Grinding Frequency: 3-30times/sec

2.Timing : 1-99 minutes

3.Rated: 375W

4.Sample Size: with four sets of accessories :0.2-0.5ml/192 holes,

1ml/72 holes,2ml/64 holes and 5ml/48 holes

5.Dimensions: L520×W365×H505 mm

6.Supplied with a hundred of steel balls for free.

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Widely used in: molecular marker-assisted breeding, genomics, systems biology and molecular evolution, transgenic research, and other biochemical research in plant tissue nucleic acid extraction.

To isolate nucleic acids from intact seeds, the seeds are first mechanically disrupted and the nucleic acids are extracted and purified. The usual mechanical breaking methods are slow and prone to cross-contamination and are not suitable for high-throughput seed breakage.

Grinding the seeds in the microplate with Scientz-48 provides a rapid release of large amounts of nucleic acid from the seed cells; the nucleic acid is separated and purified from the homogenate. For example: overnight soaking in water with water, homogenized into a slurry within 3 minutes, source of material for DNA analysis.

   Main Technical Parameters：

Power Supply：220V/50Hz

Rated Power：180W

Timing：1（S）-9999（S）

Frequency Setting：10-70Hz，300-2100times/min

Clamp Stroke：34mm（vertical）

Sample Capacity：2ml Adaptor 48hole、5ml Adaptor12 hole

Display：7inch TFT Touch Screen

Protection: Open lid protection，Emergency braking function

Dimension：L369*W360*H520mm

Accompany accessories: 500pcs 3mm stainless steel ball and 500pcs 6mm stainless steel ball

The post High-throughput Tissue Grinder For Nucleic Acid, Protein Extraction Grinding Plant Roots, Stems, Leaves, Flowers, Fruits, Seeds appeared first on Keylaser Armenia.

Rapid extraction of genomic D N A from cultured cells to prepare for P C R analysis

PCR technology improves the efficiency of nucleic acid detection and quantitative sequencing. However, a step involving cell collection and nucleic acid lysis purification is required before template amplification, and the process is slow. The nucleic acid is then separated from the lysate using a chromatography resin. It takes a lot of time and expensive materials.

Scientz-48 technology rapidly breaks up large numbers of cultured cells to prepare for PCR analysis of late genomic DNA. Scientz-48 performs high-throughput homogenization on a large number of cultured cells in microplates, and then purifies the nucleic acids by chromatography resin to perform PCR analysis, which greatly improves the efficiency of genome analysis.

Main Technical Parameters：

Power Supply：220V/50Hz

Rated Power：180W

Timing：1（S）-9999（S）

Frequency Setting：10-70Hz，300-2100times/min

Clamp Stroke：34mm（vertical）

Sample Capacity：2ml Adaptor 48hole、5ml Adaptor12 hole

Display：7inch TFT Touch Screen

Protection: Open lid protection，Emergency braking function

Dimension：L369*W360*H520mm

Accompany accessories: 500pcs 3mm stainless steel ball and 500pcs 6mm stainless steel ball

The post High-throughput Tissue Grinder Quickly Extracts Genomic D N A From Cultured Cells appeared first on Keylaser Armenia.

Yeast has become a universal host for gene expression research and recombinant expression of proteins, and has become a model organism for biological systems research and a powerful tool for biopharmaceuists. Including Picbia, Hansenula, and Debaryomyces are used by researchers such as the most common Saccbatomyces yeast. Yeast mRNA and intracellular proteins are difficult to extract by conventional enzymatic methods. Lytic enzymes usually contain ribonucleases and other proteins that attack not only the cell wall but also specific molecules. Moreover, the protoplasts produced by enzymatic hydrolysis need to be dissolved by means of specific reagents, resulting in denaturation of many proteins.

In the usual pressing or ball milling method, the yeast cells can only be broken under a single sample, and the internal solution is released, and the operation efficiency is too low. Scientz-192 designed high-throughput screening tests for large numbers of yeast clones, designed deep-well plates for broken seeds, and shredded yeast in deep-well plates. Achieve high-throughput cells.

The high-throughput tissue grinder allows for fast, efficient grinding of up to 4*96 samples simultaneously in 3 minutes. It can be dry-grinded, wet-grinded and mixed, and homogenized. In addition, special cell broken beads can break the ultrasonic and high-pressure broken cells that are difficult to break. The types of samples include: plant tissue (rice, corn, wheat, beans, peanuts, seeds, stems, roots, leaves, cotton fabrics), animal tissues (muscle, small intestine, heart, liver, lungs, fur, bones, etc.) , cells, bacteria, yeast and spores. For the extraction of nucleic acids in large-scale samples, the grinding efficiency and quality are greatly improved, the labor cost is reduced, and the labor intensity of the operator is reduced.

The post Yeast Yeast’s 9 6-well High-flux Crusher appeared first on Keylaser Armenia.

The main technical parameters

1. Time setting: 1 (seconds) – 9999 (seconds)

2. Frequency setting: 10-70Hz, that is, oscillation 300-2100 times/min

3. Rated power: 180W

4. Fixture stroke: 34mm (vertical)

5. Temperature setting: -20 ° C or -30 ° C

6. Electromagnetic lock control opens the door

7. Sample capacity: random standard: aluminum alloy adapter 5ml 12 holes, 2ml 48 holes

Optional: Aluminum adapter 2ml24 hole

8. Power requirements: 220V single phase AC, <2.5A

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