Yeast Yeast’s 9 6-hole high-throughput fragmentation
Yeast has become a universal host for gene expression research and recombinant expression of proteins, and has become a model organism for biological systems research and a powerful tool for biopharmaceuists. Including Picbia, Hansenula, and Debaryomyces are used by researchers such as the most common Saccbatomyces yeast. Yeast mRNA and intracellular proteins are difficult to extract by conventional enzymatic methods. Lytic enzymes usually contain ribonucleases and other proteins that attack not only the cell wall but also specific molecules. Moreover, the protoplasts produced by enzymatic hydrolysis need to be dissolved by means of specific reagents, resulting in denaturation of many proteins.
In the usual pressing or ball milling method, the yeast cells can only be broken under a single sample, and the internal solution is released, and the operation efficiency is too low. Scientz-192 designed high-throughput screening tests for large numbers of yeast clones, designed deep-well plates for broken seeds, and shredded yeast in deep-well plates. Achieve high-throughput cells.
The high-throughput tissue grinder allows for fast, efficient grinding of up to 4*96 samples simultaneously in 3 minutes. It can be dry-grinded, wet-grinded and mixed, and homogenized. In addition, special cell broken beads can break the ultrasonic and high-pressure broken cells that are difficult to break. The types of samples include: plant tissue (rice, corn, wheat, beans, peanuts, seeds, stems, roots, leaves, cotton fabrics), animal tissues (muscle, small intestine, heart, liver, lungs, fur, bones, etc.) , cells, bacteria, yeast and spores. For the extraction of nucleic acids in large-scale samples, the grinding efficiency and quality are greatly improved, the labor cost is reduced, and the labor intensity of the operator is reduced.
