Features:
Configuration Upgrade
Up to 3 lasers and 15 fluorescent channels are configured, with a maximum of 18 detection parameters for BioCyte-B5R3V7
Powerful Function
High resolution, high sensitivity and high stability
Automatic
Full-automatic detection process, synchronous collection/analysis
Automatic Analysis
Automatic fluorescence compensation adjustment function based on database, without changing the acquisition conditions
Absolute Counting
The absolute counting method based on the volume method of plunger pump is more accurate
Injection Options
The autosampler supports 40-tube Rotary/96-well microplate, which is simple and efficient
High sensitivity and high resolution
Fluorescence sensitivity of Rainbow calibration particles(8 peaks)
New WDM splitter module
The new WDM splitter module not only reduces the number of filters used but also reduces energy loss, further improving the sensitivity of fluorescence detection.
Highly efficient and time-saving, compatible with 40-tube rotary/96-well microplate autosampler
Software Features
Chinese and English software, easy to learn and operate, can be switched freely
Modular design, fully automatic detection, simultaneous acquisition and analysis
One-click import of templates, custom editing reports based on needs
Automatic fluorescence compensation adjustment function, no need to change acquisition conditions
• Analysis of 6-color human peripheral blood immune cell subsets
• Analysis of 9-color human peripheral blood immune cell subsets
• Detection of dendritic cells (DC)
• Phenotypic characterization of natural killer cell(NK) populations
• Characterization of PBMC T cell receptors
• Detection of CD62P Platelet Activation
Immunology
• Detection of T cell proliferation(CFSE)
• Detection of B-cell antibody class
• NK cell-mediated clearance of spleen cells
• Detection of Treg cell subsets
Stem Cells
• Flow cytometry analysis of hematopoietic stem cells from mouse bone marrow
• Detection of CAR-T extracellular tumor killing
• Analysis of mesenchymal stem cells from bone marrow and endometrium of mice
• Flow cytometry analysis of mouse hematopoietic progenitor cells
Cell Biology
• Cell activity test
• Cell cycle detection
• Cell proliferation detection
• Cell apoptosis detection
Oceanography
• Detection of quantity, size and morphology of marine microorganisms
• Detection of DNA content in marine microorganisms
• Determination of Liposome Content in Marine Microalgae
• Detection of marine bacterial activity and membrane integrity
Environmental Monitoring
• Flow cytometric detection of bacterial life and death
• Absolute count of virus, bacteria and algae
• Detection of bacterial periodic ploidy
• Algae classification and counting
Clinical
• Lymphocyte subgroup analysis
• HLA-B27 detection
• Monocyte HLA-DR detection
• Detection of neutrophil CD64
• Regulatory T cell detection
• T cell activation and diseases
• Th1 Th2 detection
• Th17 cell detection
• Blocking Antibody Detection
• Cytokine detection
• Platelet-leukocyte adhesion
• Platelet autoantibody detection
• Platelet activation and diseases
• Paroxysmal nocturnal hemoglobinuria
• Leukemia immunotyping test
• Detection of residual focus of leukemia
• MDS immune typing
• Hematopoietic stem cell count
• Lymphoma immunotyping
• Detection of cell cycle and ploidy
Flow Cytometry Laser/Channel/Fluorochrome Configuration Table
Laser Wavelength
Channel
Filter
Recommended Fluorescent Dyes
BioCyte-B5R3V7
BioCyte-B5R3
488nm
BL1
525/40
FITC, EGFP、BB515, Alexa Fluor 488
✓
✓
BL2
575/30
PE, DsRed
✓
✓
BL3
620/30
PE-Texas Red, ECD, PE-CF594, PI, 7-AAD
✓
✓
BL4
697/60
PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy5.5, BB700, PerCP-eFluor710
✓
✓
BL5
780/60
PE-Cy7, PE-Alexa Fluor 750
✓
✓
638nm
RL1
670/20
APC, Cy5, Alexa Fluor 647
✓
✓
RL2
720/40
APC-R700, Alexa Fluor 680, Alexa Fluor 700
✓
✓
RL3
780/60
APC-Cy7, APC-eFluor780, APC-H7
✓
✓
405nm
VL1
425/20
BV421
✓
VL2
460/40
Pacific Blue, V450, BV480
✓
VL3
525/40
BV51, V500
✓
VL4
605/40
BV605
✓
VL5
660/40
BV650
✓
VL6
720/40
BV711
✓
VL7
780/60
BV786, BV750
✓
Technical Parameters:
Model
BioCyte-B5R3
BioCyte-B5R3V7
Software
English/Chinese
PC
CPU i5/16G DDR4/1T hard disk+27 inches monitor
Power Supply
AC220/110V±10%, 50/60Hz
External Size(W*D*H)
530*450*470mm
Net Weight
35kg
Packing Size(W*D*H)
Main Instrument: 860*575*720mm
Accessories: 860*680*810mm
Reagent: 380*280*320mm
Gross Weight
Main Instrument: 67kg
Accessories: 53.5kg
Reagent: 16kg
Recommended Reagent Information:
Item
Recommended Reagent
Application Department
Lymphocyte Subset T Analysis
CD45
CD4
CD3
CD8
Clinical Laboratory
Infectious Disease Department
Oncology Department
Department of Rheumatology and Immunology
Department of Pediatrics Blood Specialty
Lymphocyte Subset T/B/NK Analysis
CD45
CD3
CD16
CD56
CD19
Absolute Count of CD4+T Lymphocyte
CD45
CD4
CD3
CD8
CDC (AIDS)
Infectious Disease Department
Cytokine Th1/Th2/Th17 Analysis
Th1: IL-2, IFN-γ, TNF-α
Th2: IL-4, IL-6, IL-10
Th17: IL-17
Infectious Disease Department
Oncology Department
Department of Rheumatology and Immunology
Department of Organ Transplantation
Respiratory Department
HLA-B27 Detection for AS
HLA-B27-FITC
HLA-B7-PE
IgG2a-FITC
IgG1-PE
Department of Rheumatology and Immunology
Orthopedics
DFI Analysis
Acridine orange (AO) Staining Solution
Reproductive Department
Infertility Department
Reproductive Medicine Centre
