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In order to study the role of DNA molecules in the metabolism of life, it is often necessary to extract DNA from different biological materials. Due to the different distribution and content of DNA molecules in the organism, it is necessary to select appropriate materials to extract DNA.
In animals and plants, the embryos of calf thymus, animal liver, fish sperm, and plant seeds are rich in DNA.
Among the microorganisms, glutamic acid bacteria contain 7%-10%, brewer’s yeast contains 6%, and Escherichia coli contains 9%-10%.
Different methods of extracting DNA from various materials have different degrees of difficulty in separation and extraction.
For lower organisms. It is easier to extract DNA from viruses. Most of the virus DNA has a small molecular weight and it is easy to maintain its structural integrity during extraction.
It is more difficult to extract DNA from bacteria and higher plants and animals. Bacterial DNA has a large molecular weight, typically up to 2*10 Daltons. Therefore, it is easy to be cut by mechanical tension. Bacterial DNA, in addition to nuclear DNA, plasmid DNA, etc.
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