Fluorescent Quantitative PCR Detection System

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Feature:

♦ Six independent temperature control

♦ Thermal compensation technology of temperature module

♦ No edge effect of temperature and optics have

♦ Maintenance-free long – life continuous spectral excitation source

♦ Synchronous and fast acquisition of low temperature CCD 0.15s in cold state

♦ Relative quantitative high sensitivity 1.5 times effective discrimination

♦ Flexible, open and user-friendly software platform

♦ Open reagent consumable platform

Application:

♦ Basic materials research

♦ Pathogen detection

♦ Public health safety surveillance

♦ Meat products adulterated

♦ Transgenic testing

♦ Food safety inspection

♦ Drug development and rational drug use

♦ Precise tumor treatment

♦ Gene expression

♦ Genetic screening

♦ Genotyping

♦ Stem cell research

Temperature control module

On the basis of the six independent temperature control technology, using the enclosed liquid heat transfer efficiency, combined with environmental scanning monitoring regulation auxiliary infrared with heating and intelligent variable frequency system voltage, current, ensures the module heating speed, effectively prevents temperature overshoot no edge effect and the evaporation temperature, not only saves your precious time to amplify the required temperature uniformity and the repeatability of the results achieved perfection, no matter what kind of test mode, application, it can get good data uniformity and repeatability.

Optical module

On behalf of the world’s leading optical transmission and collection technology, use optical fiber after the excitation light source for reaction system and the emission of light from the reaction system after excitation, the energy attenuation without conduction to each reaction holes and cold CCD, from physical properties to ensure consistency and authenticity of the excitation and detection, and greatly improve the detection sensitivity, can make your low effective detection and distinguish easily copy sample. The channel mismatch function is added to extend the application field of qPCR to the protein level and provide a new way for the construction of multiple systems of diagnostic reagents.

 

Channel Excitation

wavelength (nm)

Emission

wavelength (nm)

Examples of fluorescent dyes
1 Blue 460-480 512-528 FAM/SYBR Green I/Eva Green…
2 Green 515-535 562~578 VIC/JOE/HEX/TET…
3 Yellow 560~580 612~628 ROX/Texas Red …
4 Red 610-630 662~678 Cy5…
5 Red 660-680 702~718 Cy5.5/Quasar705…

 

♦ Channel combination: 6-color excitation light filter and 6-color detection light filter can detect≥21 different fluorescence spectra, channels are completely open, supporting third-party reagent optimization Support FRET application.

♦ Multichannel static fluorescence function: analysis of starting template and end product.

♦ Filter can be customized according to customer need

 

Software platform

Real-time monitoring, automatic discrimination and calculation of positive and negative results, automatic establishment of standard curve, absolute/relative quantitative, multiple quantitative, fusion curve, gene mutation, quality control graphic analysis, fluorescence correction, PCR amplification efficiency, high-resolution fusion curve (optional match), etc.

Models:

Technical parameters:

Product Name Fluorescence Quantitative PCR Detection System
Model MA-6000
Sample Capacity 96×0.2ml PCR plate, 12×8-strip tubes, 96*0.2ml single tube
Reaction System 6~125μl
Dynamics Range 1-1010 copies
Channel 5
Emission Light Full-wavelength maintenance-free halogen tungsten lamp
Detector -20℃ CCD
Detection Path F1 F2 F3 F4 F5
Suitable Probe/Dye FAM/SYBR Green/Eva Green/LC Green/Fluorescein VIC/HEX/TET/Cy3/JOE/Alex a555 ROX/Cy3.5/Texas Red Cy5/LC Red640 Cy5.5/LC Red705
Excitation Wavelength 380~780nm
Detection Wavelength 380~780nm
Fluorescence Detection Repeatability CV≤3%
Fluorescence Detection Precision CV≤ 5%
Sample Detection Repeatability CV≤3%
Fluorescence Detection Linearity r≥0.980
Confidence It can effectively distinguish 5000 and 1000copies with99.8% confidence degree
Module Temp. Range 4-100℃(resolution:0.1℃)
Heating Speed 5.0℃/s(Max), 3.5℃/ s(Avg)
Cooling Speed 5.0℃/s(Max), 3.2℃/ s(Avg)
Temp. Accuracy ±0.1℃
Temp. Uniformity ≤ ±0.25℃
Number of Temp. Control Areas 6

 

Gradient Temp. Range 1-32℃
Hot-Lid Temp. Range 104℃, Automatic Hot-lid
Special Function Absolute quantification, Relative Quantification, Genotyping, Amplification efficiency calculation, Melting curve.
Operation System Windows 7 64-bit or higher
Power Supply 100~240V,50~60Hz
Dimension(L*W*H) mm 600*390 *320
Port Method USB Port
Packing Size(L*W*H) Mm 850*600 *650
Gross Weight 51

 

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