Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

LEIA-X4 LEIA-X6

Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Application:

It can be widely used in infectious disease research, food pathogen detection, waterborne pathogen detection, drug analysis, stem cell research, pharmacogenomics research, tumor and genetic disease research, plant science and agricultural biotechnology.

Working Principle:

The temperature step change is controlled by the semiconductor Peltier to realize PCR amplification. Use high-sensitivity PD unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control X and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software.

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6
Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

 

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6
Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

 

Features:

1. Excellent temperature control performance of the instrument, Module Max heating rate heating rate 7.0℃/s.

2. No edge effect, no optical path correction, top excitation/detection, non-contact measurement.

3. Fluorescence detection adopts PD sensor with high sensitivity.

4. Long-life LED light source, stable emission wavelength, maintenance-free.

5. 4/6-channel fluorescence detection, no cross interference between channels.

6. User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored.

 

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6
Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

 

Real-time PCR Hardware:

Thermoelectric cooling module (TEM) is a semiconductor device composed of many tiny and effective heat pumps. By applying a low-voltage DC power supply, heat will be transferred from one side of the TEM to the other side, resulting in a phenomenon that one side of the TEM becomes hot and the other side becomes cold. Since this phenomenon is completely reversible, when the polarity of the DC power supply is changed, it will be the opposite. This product adopts a long-life series TEM, which provides longer life and more efficiency during thermal cycling.

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6 

The integrated design of the scanning module and the heating cover module, relying on its own gravity to compress the heating plate and the reagent cover, and is supported by four compression springs to prevent the sample tube from being crushed by four; at the same time, the rubber pad around the heating cover is pressed to ensure that there is no external light source interference in the detection ; The bottom of the cam mechanism is used to support the space to ensure the smooth sliding of the heating module; the guide rail mechanism on both sides of the heating module prevents the module from shifting and ensures the accuracy of the mechanical scanning structure.

 

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6
Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

 

The software includes functions such as absolute quantification experiment, melting curve experiment, relative quantification (AACT) experiment, and genotyping experiment. Enter the attribute setting interface and select different function modules. Guided flow operation, convenient for users to quickly complete experimental settings. The software can open the recent experiment record template for easy viewing and the creation of new experiments.

 

Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6
Fluorescent Quantitative PCR Detection System LEIA-X4 LEIA-X6

 

Technical Parameters:

Model LEIA-X4 LEIA-X6
Sample Capacity 96*0.1ml PCR plate, 12*8-strip tubes, 96*0.1ml single tube (Transparent Cover)
Reaction System 5~50μl
Dynamics Range 1~10¹⁰ copies
Channel 4 6
Emission Light LED
Detector PD
Detection Path F1: FAM/SYBR GREEN;

F2: VIC/JOE/HEX/TET;

F3: ROX/TEXAS-RED;

F4: Cy5

F1: FAM/SYBR GREEN;

F2: VIC/JOE/HEX/TET;

F3:CY3;

F4: ROX/TEXAS RED;

F5: CY5;

F6: CY5.5

Excitation Wavelength 455~650nm 455~685nm
Detection Wavelength 510~710nm 510~740nm
Fluorescence Detection Repeatability CV≤3%
Fluorescence Detection Accuracy CV≤5%
Fluorescence Detection Linearity r≥0.995
Module Temp. Range 4~105℃
Ramp Rate 7.0℃/s(max)
Temp. Accuracy ±0.5℃
Temp. Uniformity ≤±1℃
Temp. Control Mode Block and tube
Gradient Temp. Range It can be set within the temperature range of 30°C to 105°C, with a temperature gradient span of 1°C to 36°C
Hot-Lid Temp. Range 100℃, automatic hot-lid
Scanning Mode Top scanning
Operation Mode Continuous
Scanning Time 8.5s
Special Function Absolute quantitative automatic analysis, relative quantification, SNP Analysis, melting curve analysis, etc.
Operation System Microsoft: Windows 10
Port Method USB Port
Power Supply AC100~240V, 50/60Hz
Dimension(W*D*H) 375*505*375mm
Net Weight 28kg
Packing Size(W*D*H) 645*565*605mm
Gross Weight 49kg

 

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