The DNA cleavage system, also known as DNA fragmentation, refers to the fragmentation of intact long-chain DNA molecules into small-molecule DNA fragments ranging in length from 100 bp to 1000 bp by physical or chemical means.
Common methods:
Ultrasonic disruption, restriction enzyme digestion, kit
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Technical principle
The vibration of mechanical waves between 20 Hz and 20 kHz forms acoustic waves in the elastic medium. The ultrasonic waves between 20 kHz and 500 MHz are called ultrasonic waves. The propagation speed of ultrasonic waves is the propagation speed of sound waves, while the ultrasonic waves have short wavelengths, which are easy to directional emission and convergence. advantage. Using geometrically focused sonic energy, the 0.5MHz spherical solid-state ultrasonic sensor focuses the acoustic energy at a wavelength of 1mm onto the sample, not only controlling the waveform, but also auto-focusing energy without loss and acting directly on the sample inside the tube.
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Feature
| Ultrasonic frequency: 40 kHz, automatic frequency tracking
Temperature control: external low temperature thermostat, more precise temperature control Ultrasonic power: 300 W, 40%-100% adjustable Wave method: the bottom of the sink wave, the ultrasonic dispersion is more uniform Interrupted fragment size: 200 bp-1000 bp Appearance size (length * width * height): 40 * 26 * 29 cm Body weight: 12 kg |
| Sample rack specifications and throughput:
0.2-0.5ml 12 wells, throughput: 5ul-150ul 1.5-2ml 6 wells, throughput 100ul-800ul 5ml 6 holes, processing capacity 500ul-3ml 6-12 samples can be processed at one time, different adapters are selected for different sample volumes |
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