The high-pressure homogenizer is a commonly used device, which consists of a positive displacement pump and a discharge valve that can generate high pressure, and the discharge valve has a narrow small hole, and its size can be adjusted. The cell slurry enters the pump body through the check valve, and under high pressure, it is forced to rush out in the small hole of the discharge valve and hit the impact ring. Due to the sudden decompression and high-speed impact, the cells are subjected to high liquid shear. Breaking force and breaking.
In the operation mode, it can be operated by a single pass through a homogenizer or repeated cycles, or continuously. In order to control the temperature rise, the temperature can be adjusted at the inlet with dry ice to adjust the outlet temperature to about 20 °C.
In the industrial cell disruption, repeated cycling operations are often used for cells that are difficult to break, such as yeast, and which are high in concentration or in the stationary phase of growth.
Technical Parameters
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