Biolistic Gene Gun

Gene gun technology, also known as particle bombardment, is an advanced gene – transfer technique. In this process, micron – sized gold or tungsten particles coated with nucleic acids are accelerated to the appropriate velocity. This enables the introduction of genes into cells, tissues, and organs, achieving rapid, stable, and highly efficient expression and transformation. This technology is characterized by its speed, ease – of – use, and safety. Our gene gun product has received notable accolades: It was awarded the Second Prize of Zhejiang Provincial Scientific and Technological Progress. It also won the Second Prize of Ningbo Municipal Scientific and Technological Progress.

Working Principle

The rupturable membrane is made of polyimide films of different thicknesses to regulate the helium pressure. When the helium pressure reaches the critical pressure of the rupturable membrane, the rupturable membrane bursts and releases a powerful shock wave, which causes the particulate bullet carrier to carry the particulate bullet downwards at high speed to bombard the target cells or tissues.

When the helium pressure reaches the critical pressure of the rupturable membrane, the rupturable membrane bursts and releases a powerful shock wave, which causes the particle bullet carrier to carry the particle bullets downward at a high speed and bombard the target cells or tissues.

System Standard Configuration

Main machine, vacuum pump, 1000 guns consumables (3.5/4.5/7Mpa rupturable membrane, carrier membrane, stainless steel mesh, 2 bottles of gold powder, 1 bottle of tungsten powder)

Gene gun System Standard Configuration

Features

High Versatility : Gas-driven design: Easier operation + wider applications

High Conversion Rate: Biologically inert vectors enhance safety and precision

Quick Cleaning: Detachable circular chamber for easy sterilization

Pressure Adjustable: Adjustable and optimised between 3-12 MPa.

Optional Carrier Membranes: Flying or steel membranes are available depending on the receptor.

Low cost: About $18 per shot except for high pressure gases

Application

Food Industry

Agricultural and Forestry Sciences

Biological Engineering

Animal Medicine

– Research on transient manifestations of gene function;

– Research on manifestations of functional stability;

-Genetic or cancer-related research;

– DNA vaccine research and application;

– Gene therapy related research

– Transgenic plant research;

– Genetic engineering or drug-related research

Technical Parameters
Target Area Maximum 40cm²
Working Pressure Range 3-12MPa
Target Type

 

 

Animals: cell and organ culture
Plants: smaller whole plants, cultured cells, exosomes
Yeast, bacteria, other microbial cells, chloroplasts, mitochondria
Voltage 220v/50hz
Air Source Helium or nitrogen (helium is more optimized)
Metal Particles Φ0.8-1.5μm; Φ1.5-3.0μm
Scroll to Top