Gene Gun Biolistic is equipped with a unique mechanism for generating gas shock waves. It can produce gas pressures ranging from 0.5 Mpa to 5 Mpa. By utilizing helium or nitrogen as the power source, it generates a “cold” gas shock wave. This shock wave is then employed to transport DNA micro – bombs into the receptor.
With helium or nitrogen as the power source, the Gene Gun Biolistic creates a “cold” gas shock wave that effectively delivers the DNA micro – bomb to the receptor. This approach avoids cell damage that might be caused by a “hot” gas shock wave, enabling the completion of high – quality transgenic work.
Features
High Conversion Rate
Uniform dispersion + convertible receptor
Functionality
Realise the transformation of cells with special growth needs.
Convenient,
portable and hand-held design for on-site transformation.
High Specificity
No introduction of unrelated genes or proteins into the sample
Low Sample Requirements
Low-input, vector-free, size-unlimited DNA processing
Technical Parameters
| Experimental conditions | In situ, in vitro, in vivo |
| Target area | (small) 2cm² |
| Working Pressure Range | 0.5-5 MPa (12 times per revolution of the magazine) |
| Target type | Animal cells, plant cells, living plant leaves, |
| Voltage | Live animal skin, muscle, live animal organs |
| Gas source | 9V (can be fired 1000 times) |
| Metal particles | Helium or nitrogen |
| Bombardment method | Φ0.8-1.5μm/Φ1.5-3.0μm |
Reference table of experimental conditions
| Receptor Name | mice | Rats |
| Firing site | Abdomen | Liver or kidney |
| Pressure | 2.54MPa | 1-1.5MPa |
| Gas | Helium | Helium |
| Shooting Distance | Launch tube tightly attached | 1-3mm |
| Metal particles | φ1.6μ gold or tungsten | φ1.6μ gold or tungsten |
| Remarks | Fudan University Military Academy | Biophysical Institute |
